Propolis or bee glue is a resinous mixture that honey bees produce by mixing saliva and beeswax with exudate gathered from tree buds, sap flows, or other botanical sources. It is used as a sealant for unwanted open spaces in the hive.
Propolis is used for small gaps (approximately 6 millimeters (1⁄4 in) or less), while larger spaces are usually filled with beeswax. Its color varies depending on its botanical source, with dark brown as the most common. Propolis is sticky at and above 20 °C (68 °F), while at lower temperatures it becomes hard and brittle.
When foraging, worker bees primarily harvest pollen and nectar, while also collecting water and tree resin necessary for the production of propolis.The chemical composition and nature of propolis depend on environmental conditions and harvested resources.
For centuries, beekeepers assumed that bees sealed the beehive with propolis to protect the colony from the elements, such as rain and cold winter drafts. However, 20th-century research revealed that bees not only survive, but also thrive, with increased ventilation during the winter months throughout most temperate regions of the world.
Propolis properties may include:
- reinforce the structural stability and provide thermal insulation to the hive
- reduce vibration
- provides anti-fungal and antibacterial properties in the nest
- make the hive more defensible by narrowing the existing entrance (in wild colonies) to a single “choke point”
- make the hive more defensible by sealing holes: a hive will have a cache of as much as 1 pound (0.45 kg) of propolis for emergency patch jobs
- prevent diseases and parasites from entering the hive, and to inhibit fungal and bacterial growth
- mitigate putrefaction within the hive. Bees usually carry waste out of and away from the hive. However, if a small lizard or mouse, for example, finds its way into the hive and dies there, bees may be unable to carry it out through the hive entrance. In that case, they would attempt instead to seal the carcass in propolis, essentially mummifying it and making it odorless and harmless.
In neotropical regions, in addition to a large variety of trees, bees may also gather resin from flowers in the genera Clusia and Dalechampia, which are the only known plant genera that produce floral resins to attract pollinators. Clusia resin contains polyprenylated benzophenones.
In some areas of Chile and Argentina Andean valleys, propolis contains viscidone, a terpene from Baccharis shrubs, and in Brazil and Paraguay, naphthoquinone epoxide has recently been isolated from red propolis, and prenylated acids such as 4-hydroxy-3,5-diprenyl cinnamic acid have been documented.
An analysis of propolis from Henan, China found sinapinic acid, isoferulic acid, caffeic acid, and chrysin, with the first three compounds demonstrating antibacterial properties.Also, Brazilian red propolis, largely derived from Dalbergia ecastaphyllum plant resin, has high relative percentages of the isoflavonoids 3-hydroxy-8,9-dimethoxypterocarpan and medicarpin.
Other flavonoids commonly present include galangin and pinocembrin. Caffeic acid phenethyl ester (CAPE) is also a component of some varieties of propolis from New Zealand.
Occasionally, worker bees will even gather various caulking compounds of human manufacture, when the usual sources are more difficult to obtain.
The properties of the propolis depend on the exact sources used by each individual hive; therefore any potential medicinal properties that may be present in one hive’s propolis may be absent from another’s, or from another sample in the same hive.
Traditional medicine and research
Propolis has been used in traditional medicine.There is insufficient evidence to rate the effectiveness of propolis for any condition.
The propolis made by some Brazilian bees is under preliminary research for the potential development of new drugs associated with control of Candida albicans and immunomodulatory effects.Studies have suggested propolis may be effective treatment for allergic rhinitis by inhibiting histamine release.
Propolis is used by some string instrument makers (violin, viola, cello, and bass) as a varnish ingredient. A tincture of propolis may be used to seal the surface of newly made violin family bridges, and may be used in the maintenance of the bores of pan flute tubes. Propolis was purportedly used by Antonio Stradivari in the varnish of his instruments.
Propolis is used by some chewing gum manufacturers to make propolis gum.
Propolis is used to bring about a chemical reaction to convert fats and oils into automobile wax during application.